Characterized Bacteria Resources from Clinical Specimen in Korea, 2014-2016


Department of Laboratory Medicine, College of Medicine, Yonsei University Kim Dokyun, Jeong Seok Hoon, Lee Kyungwon Pathogen Resource TF, Center for Infectious Disease, NIH, CDC Chung Hyangmin, Hwang Kyu Jam

Background: The pathogen resources bank for the clinical isolates became an important issue especially after the Nagoya Protocol in 2014. However, the previous system for the bacterium-banking process had limitations including lack of detailed information about the species identification, minimal inhibitory concentration, and mode of resistance. We isolated, characterized, and deposited clinical isolates from the National Culture Collection for Pathogens (NCCP) from 2014 to 2016. Methodology: Survey about the stock and demand of the reference strains was given to the experts of the Korean Society of Clinical Microbiology. Referring to the result of the survey, we collected useful or rare clinical isolates in the tertiary hospitals from 2014 to 2016. The collected isolates were characterized in terms of identification, phenotype and genotype of resistance and toxin, and strain type.

Results: We have deposited 416 strains in the bank to characterize bacteria resources. A total of 193 strains were allotted for species identification, 40 strains for antimicrobial susceptibility testing, 14 strains for toxin gene testing, 169 strains for research of epidemiology and mode of resistance. These strains would expand the resources of the National Culture Collection for Pathogen and would also be useful for future medical research and the development of identification devices.