Library construction techniques for pathogen whole genome sequencing

Yang Hyo-Jin, Choi Hyun-Jung, Chai Hee-Youl, Kang Yeon-Ho*
Division of Biosafety Evaluation and Control, KNIH, KCDC

In molecular biology, libraries are widely used for analyzing the sequences of gene or genome to discover a new gene and its function. Furthermore, libraries are essential in genomics for preparing genetic materials into a form compatible with the sequencing system to be used. For this reason, the library preparation method has evolved from that applied in first-generation sequencing techniques (Sanger sequencing) to more recent sequencing technology [second- generation sequencing i.e., next-generation sequencing (NGS) and third-generation sequencing]. Moreover, the methods are changed depending on the type of target genetic materials (e.g., DNA, RNA). In this report, we introduce general library preparation methods and specific library construction methods according to the type of pathogens being detected.

Keywords: Library construction, Pathogen, Whole genome sequencing, Genome, Genetic material